First Case of the Isolation of the H3n2 Swine Influenza Virus in Poland
نویسندگان
چکیده
The aim of the study was the molecular characterisation of the aetiological agent responsible for the outbreak of swine influenza with mild respiratory tract disorders, which occurred in piglet sector in 2008. RT-nested-PCR for the detection of the conservative region of the matrix 1 gene and subsequently multiplex PCRs were performed to detect HA and NA genes. The amplified mPCR products were sequenced and the phylogenetic alignment was analysed. The new strains isolated from the outbreak were determined as H3N2. They represent the initial newly emerged subtype in our country, and the second one in the CentralEastern part of Europe. The new Polish isolates were most similar to the new evolutionary SIV strains from Spain, Belgium, and Italy.
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